2 hours
I provide professional and reproducible bioinformatics analysis of DNA methylation sequencing data for researchers, laboratories, and institutions. I can analyze EM-seq, whole genome bisulfite sequencing, RRBS, targeted bisulfite sequencing, methylation arrays, or processed methylation datasets. Depending on your project, the service may include: • Raw FASTQ quality control • Adapter and quality trimming • Alignment to the reference genome • Methylation extraction and coverage assessment • Sample quality and exploratory data analysis • Differentially methylated cytosine analysis • Differentially methylated region analysis • Genomic and gene annotation • Pathway and functional enrichment analysis • PCA, clustering, heatmaps, volcano plots, and publication-ready figures • Biological interpretation of the results • Reproducible analysis scripts • A clear scientific report summarizing the workflow, results, and conclusions The workflow will be adapted to your organism, experimental design, sample size, biological comparisons, and research objectives. Before starting, I will review your project requirements, available files, metadata, reference genome, comparisons, and expected deliverables. Please contact me before ordering projects involving large sequencing datasets or customized analyses. Google Scholar: https://scholar.google.com/citations?hl=en&user=dqTWxUwAAAAJ
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